nanotechnology journal

Multifunctional Nanoplatform for Targeted Laser-induced Hyperthermia and Microscopy of Breast Cancer Cells using SPION-based Gold and Folic Acid Conjugated Nano dendrimers: An in vitro Assay

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The use of nanotechnology seems to be a promising approach for early detection, imaging and therapy of cancer. In this investigation, 9 nm superparamagnetic iron oxide nanoparticles (SPION) were synthesized and functionalized by third generation polyamidoamine (G3-PAMAM) dendrimer. The magneto dendrimer samples were then conjugated by folic acid molecules for targeting and by gold nanoparticles for photothermal hyperthermia purpose. The gold nanoparticles were synthesized using three reducing agents of sodium borohydride, hydrazine sulfate and pre synthesized 10-nm AuNPs.

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The final nanocomposite is named IDAF NC (Iron oxide-Dendrimer-Gold-Folic acid). Two breast cancer cell lines of MDA MB 231 and MCF 7 were used for cytotoxicity, uptake and photothermal assay. The results confirmed that AuNPs prepared by sodium borohydride and the pre-synthesized 10-nm AuNPs were larger and those prepared by hydrazine sulfate were smaller and uniformly entrapped within dendrimer cavities. The MDA MB 231-IDAF-Hydr combination exhibited the highest viability percentage at 50 μg/mL before laser irradiation and lowest cancer cell viability of 65% (i.e., highest cell apoptosis and crystallization after irradiation).

Estimation of Metformin in Bulk Drug and in Formulation by HPTLC

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A simple and sensitive, HPTLC method has been developed for the quantitative estimation of metformin in its single component tablet formulation. Metformin was chromatographed on silica Gel 60 F 254 TLC plate using ammonium sulfate (0.5%): 2-propanol: methanol in the ratio of 8.0:1.6:1.6 (v/v/v) as mobile phase.

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Metformin showed R f value of 0.50±0.03 was scanned at 238 nm using Camag TLC Scanner 3. The linear regression data for the calibration plot showed a good relationship with r =0.999. The method was validated for precision and recovery. The limits of detection and quanti fi cation were 95 and 200 ng/spot respectively. The developed method was successfully used for the assay of metformin tablet formulations. The method is simple, sensitive and precise; it can be used for the routine quality control testing of marketed formulations.

Estimation of Metformin in Bulk Drug and in Formulation by HPTLC

Posted on

A simple and sensitive, HPTLC method has been developed for the quantitative estimation of metformin in its single component tablet formulation. Metformin was chromatographed on silica Gel 60 F 254 TLC plate using ammonium sulfate (0.5%): 2-propanol: methanol in the ratio of 8.0:1.6:1.6 (v/v/v) as mobile phase. Metformin showed R f value of 0.50±0.03 was scanned at 238 nm using Camag TLC Scanner 3.

Metformin in Bulk Drug

The linear regression data for the calibration plot showed a good relationship with r =0.999. The method was validated for precision and recovery. The limits of detection and quanti fi cation were 95 and 200 ng/spot respectively. The developed method was successfully used for the assay of metformin tablet formulations. The method is simple, sensitive and precise; it can be used for the routine quality control testing of marketed formulations.

Can AFM Reveal Global Viral RNA Structure?

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The genomes of RNA viruses often contain RNA structures that are crucial for translation and RNA replication and may play additional, uncharacterized roles during the viral replication cycle. RNA structure with single-nucleotide resolution. In combination with orthogonal evolutionary analyses, we uncover several conserved RNA structures in the open reading frame of the viral genome. The genomes of RNA viruses, such as poliovirus (PV), often contain complex RNA secondary and tertiary structures.

Viral RNA Structure

These structures are crucial for translation and replication of the viral genome and may play additional roles in other processes, such as genome packaging and modulation of the host antiviral response. We have used SHAPE to interrogate authentic full-length poliovirus genomic RNA extracted, in a gentle and non-denaturing manner, from purified virions. We identified many regions of the poliovirus genome where SHAPE data indicate that nucleotide flexibility is constrained, suggesting that the RNA is folded into secondary and/or tertiary structures. We then complemented our chemical probing with a parallel, independent evolutionary analysis of pairing probability, which demonstrated that several of the newly identified structures are also highly conserved.

Nanotechnology: The Risks and Benefits

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Nanotechnology is being actively developed for many applications in the medical field, including drug delivery, biosensors and medical imaging. These nanomaterials are being advanced as novel and more targeted treatments for difficult to manage diseases such as cancers. Other materials are being developed as alternatives to conventional antibiotics in treating infections.

Nanotechnology

The use of engineered nanomaterial’s (nanoparticles) offer the ability to transport therapeutics to specific sites of a disease, thus reducing the off target toxicity of many drugs. This is especially true in the use of chemotherapeutics where off target reactions cause serious side effects in cancer patients. Additionally, the field of medical imaging can be improved with the ability for the specific targeting of diseased tissues at resolutions not capable with current technologies.

Nanorobotics

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Nanorobotics is the technology of creating robots or machines at or close to the scale of nanometer. Nanorobotics refers to the nanotechnology engineering of designing and building nanorobots. Nanomachines are largely in the research and development phase. Nanorobotics refers to the still largely theoretical nanotechnology engineering discipline of designing and building nanorobots.

Nanorobotics

A nanorobot is a tiny machine designed to perform a specific task or tasks repeatedly and with precision at nanoscale dimensions, that is, dimensions of a few nanometers (nm) or less, where 1 nm = 10-9 meter. Nanorobots have potential applications in the assembly and maintenance of sophisticated systems. Nanorobots might function at the atomic or molecular level to build devices, machines, or circuits, a process known as molecular manufacturing. A major advantage of nanorobots is thought to be their durability.

The Risks and Benefits for Medical Diagnosis

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Nanotechnology is being actively developed for many applications in the medical field, including drug delivery, biosensors and medical imaging. These nanomaterials are being advanced as novel and more targeted treatments for difficult to manage diseases such as cancers. Other materials are being developed as alternatives to conventional antibiotics in treating infections.

medical-diagnosis

The use of engineered nanomaterials (nanoparticles) offer the ability to transport therapeutics to specific sites of a disease, thus reducing the off target toxicity of many drugs. This is especially true in the use of chemotherapeutics where off target reactions cause serious side effects in cancer patients. Additionally, the field of medical imaging can be improved with the ability for the specific targeting of diseased tissues at resolutions not capable with current technologies.